Croatia will be looking to secure qualification for the knockout stages of Euro 2016 when they lock horns with the Czech Republic on Friday.
Vatreni kicked off their European Championship campaign on a winning note as Luka Modric's 42nd-minute strike sealed a 1-0 victory over Turkey in their Group D opener on Sunday.
They moved level with defending champions Spain at the top of their pool on three points and have the chance to progress to the last 16 with a game to spare.
Meanwhile, Pavel Vrba's side were just three minutes away from holding La Furia Roja to a goalless draw on Monday before Gerard Pique popped up with a late winner.
The Czechs need to win at Stade Geoffroy-Guichard to keep alive their hopes of making it out of the group phase, but they have yet to beat the Croats in two previous attempts.
However, this is the first time the two nations will meet at a major tournament, and Croatia boss Ante Cacic is wary of the threat posed by Czech Republic in Saint-Etienne.
"Of all our group stage games, I dread the one against the Czechs the most," Cacic told a press conference.
"I have the utmost respect for them and we have to be razor-sharp because a last-16 spot is at stake."
In team news, Vrba has reported no new problems in his squad following their clash with Spain and will have all 23 players available for selection.
Cacic could make changes to his starting line-up, though, after Verdun Corluka sustained a head injury against the Turks with the defender requiring stitches.
"We have to prepare ourselves for a fast-paced game and it wouldn't be a drawback if we made some changes to the starting 11," Cacic told UEFA.
But skipper Darijo Srna is expected to feature, having returned to the camp after attending his father's funeral back home this week.