World number one Angelique Kerber suffered a first round loss to Ekaterina Makarova at Roland Garros on Sunday while Petra Kvitova advanced.
The top seed was sent packing in straight sets by the Russian, picking up her fifth career win over Kerber with a comprehensive 6-2, 6-2 victory on the clay courts of Paris.
The result continues Kerber's poor run of form, that has seen her fail to reach a final of any event in 2017.
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Makarova broke the two-time grand slam winner in her opening service game and consolidated to go 3-1 up. Another break would see the world number 40 take a commanding 4-1 lead, which she never relinquished.
The second set was much like the first with a plethora of unforced errors from Kerber – she would make 25 unforced errors – leading to a 4-1 deficit.
In a marathon final game, Makarova would fight off seven break points to finally hold serve and reach the second round for the fifth time in Paris.
Meanwhile, earlier in the day, Kvitova opened proceedings on Philippe-Chatrier and was too good for Julia Boserup, needing 74 minutes to roll over the American 6-3, 6-2.
It was a great result for the Czech, who has spent months on the sidelines recovering from a left hand injury sustained from fending of a burglar in her home.
In other results, Madison Brengle edged Julia Goerges in a near-three-hour marathon, winning 1-6, 6-3, 13-11 while Olympic gold medalist Monica Puig beat veteran Roberta Vinci 6-3,3-6, 6-2.
All the other results from Sunday can be seen below:
Dominika Cibulkova (Slovakia) beat Lara Arruabarrena Vecino (Spain) 6-2 6-1
Oceane Dodin (France) beat Camila Giorgi (Italy) 6-2 6-3
Bethanie Mattek-Sands (U.S.) beat Evgeniya Rodina (Russia) 7-5 6-2
Venus Williams (U.S.) beat Wang Qiang (China) 6-4 7-6(3)
Kurumi Nara (Japan) beat Amanda Anisimova (U.S.) 3-6 7-5 6-4
Cagla Buyukakcay (Turkey) beat 22-Mirjana Lucic-Baroni (Croatia) 6-3 6-3
Lesia Tsurenko (Ukraine) beat Kateryna Kozlova (Ukraine) 6-4 6-0
Svetlana Kuznetsova (Russia) beat Christina McHale (U.S.) 7-5 6-4
Shelby Rogers (U.S.) beat Marina Erakovic (New Zealand) 7-6(4) 6-4
Jelena Ostapenko (Latvia) beat Louisa Chirico (U.S.) 4-6 6-3 6-2
Timea Bacsinszky (Switzerland) beat Sara Sorribes Tormo (Spain) 6-1 6-2
Petra Kvitova (Czech Republic) beat Julia Boserup (U.S.) 6-3 6-2