The Court of Arbitration for Sport (CAS) has rejected Valentino Rossi's appeal against his penalty for this weekend's MotoGP finale at Valencia.
The Yamaha rider took his case to CAS when his appeal against the penalty of having to start of the back of the grid following a clash with Marc Marquez in Malaysia was rejected.
The CAS hearing took place on Wednesday, after his team-mate and title rival Jorge Lorenzo was denied the chance to testifie.
"Following the hearing, the CAS issued an order dismissing the application for a stay filed by Mr Rossi concerning the decision rendered on October 25 2015 by the FIM stewards," a statement from motorcycle racing governing body the FIM read.
"The FIM takes note of the ruling of the CAS.
"As a consequence of this ruling, at the Gran Premi de la Comunitat Valenciana, Mr Rossi will start from the last position on the grid."