Southampton will look to make home advantage count when they welcome FC Midtjylland to St Mary's for the first leg of their Europa League play-off tie.
Ronald Koeman's side, who qualified for the competition by virtue of their seventh-placed finish in the Premier League last season, set up a clash with the Danish champions after beating Vitesse in the third qualifying round.
The Saints ran out 3-0 victors against the Dutch outfit on home soil before securing a 2-0 win in the Netherlands to progress 5-0 on aggregate over the two legs, and they are now playing for a place in the group stages.
The Wolves, meanwhile, won the Danish Superliga title for the first time in their history last term to clinch a place in the Champions League third qualifying round, but their adventure was brief as Jess Thorup's team lost to APOEL on away goals.
Koeman can include Steven Caulker in his preparations for Thursday's game, with the on-loan QPR centre-back having recovered from a sinus infection.
New signing Oriol Romeu is also available to make his first start for the south coast club, having been handed his debut in Saturday's 3-0 defeat to Everton as a substitute.
However, the Dutchman is still without injured trio Fraser Forster, Ryan Bertrand (both knee) and Jordie Clasie (hamstring).
For the visitors, Morten Rasmussen is expected to be brought back into the starting line-up after the former Celtic striker was only amongst the substitutes for the goalless draw against FC Copenhagen last week.