The Arsenal midfielder has been out of action since last November when he injured an ankle against Manchester United at the Emirates Stadum.
He played the full 90 minutes against Stoke U21s last week, and got another 88 minutes under his belt – coincidentally against Reading U21s – in north London on Monday evening and he could now be recalled to the first-team squad for Saturday’s cup clash at Wembley.
Abou Diaby and Serge Gnabry were other Gunners continuing their comeback from injury in a game which Reading won 1-0 thanks to an eighth-minute headed goal by Niall Keown – the son of former Arsenal centre-back Martin.
Diaby played for an hour while Gnabry was given the full 90 minutes in front of 2,217 fans at the Emirates.
Wilshere, who skippered the team, enjoyed a strong second half and twice came close to scoring.